The examining committee, chaired by Assistant Professor Dr. Mustafa Saleh Ali from the College of Medicine at Al-Mustansiriya University, and consisting of Assistant Professor Dr. Taghreed Khaled Hamad from the College of Engineering at the University of Nahrain, Assistant Professor Dr. Sufian Mondir Saleh from the Biomedical Engineering Department at the College of Engineering, University of Nahrain, and Dr. Alaa Ayed Jaber from the Biomedical Engineering Department at the College of Engineering, University of Nahrain, under the supervision of Professor Dr. Jamal Abdul Jabbar Hassan from the Biomedical Engineering Department at the College of Engineering, University of Nahrain, and Dr. Iman Ghadban Khalil from the Biomedical Engineering Department at the College of Engineering, University of Nahrain. It is worth noting that the thesis was scientifically evaluated by the first scientific evaluator, Assistant Professor Dr. Zainab Wahbi Abdul Latif from the College of Medicine, Al-Mustansiriya University, and the second scientific evaluator, Assistant Professor Dr. Nibras Hussein Ghaib from the College of Engineering, Khuarizmi University of Baghdad. Linguistically, it was evaluated by Dr. Samar Ali Jaber from the Biomedical Engineering Department at the College of Engineering, University of Nahrain.

This study aims to provide a general overview of the use of the acousto-optic system by integrating low-intensity pulsed ultrasound (LIPUS) with a frequency of 1 megahertz and spatial-peak temporal-average intensity (ISPTA) of 1 watt/cm² for 90 seconds, along with low-level laser therapy (LLLT) using Nd:YAG laser with wavelengths of 532 and 1064 nanometers, pulse numbers ranging from 1 to 6, and energy range from 100 to 900 millijoules for treating the line of human breast cancer cells (MDA-MB-231). These cells were cultured in 96-well plates and treated first with low-intensity ultrasound waves, then exposed to Nd:YAG laser.

The experimental results were satisfactory in various criteria for strategies (ultrasound, laser, and acousto-optic) as cancer treatments compared to any technique alone. The results showed that the ultrasound treatment strategy was the most effective in reducing the viability of cancer cells, unlike the laser treatment strategy alone, which was the least effective. The response of cells to the effect of acousto-optical strategies was moderate between the response of cells to ultrasound and laser. In the acousto-optical system, ultrasound waves cause slight damage to the cell membrane and pave the way for the laser, which in turn accurately reaches the lesion and destroys the cells. Thus, the acousto-optical system can improve the efficiency of the system against tumor cells and is safe for the surrounding tumor tissue, eliminating the damage that may result from using ultrasound or laser treatment strategies alone. Continued research is expected to lead to the development of new and innovative methods to harness this effect clinically.

The thesis has been accepted as meeting the requirements for the Master's degree.